Robert W. Blakesley, Ph.D.

Director, Sequencing Group, NIH Intramural Sequencing Center []
Associate Investigator, NIH Intramural Sequencing Center (NISC)

Selected Publications

Boezi J.A., Lee L.F., Blakesley R.W., Koening M., and Towle H.C. Marek's disease herpesvirus - induced DNA polymerase. J. Virology, 14:1209-1219. 1974. [PubMed]
Blakesley R.W. and Boezi J.A. A kinetic and structural characterization of adenosine 5'-triphosphate:ribonucleic acid adenylyltransferase from Pseudomonas putida. Biochem Biophys Acta, 414:133-145. 1975. [PubMed]
Blakesley R.W., Dodgson J.B., Nes I.F. and Wells, R.D. Duplex regions in single-stranded FX174 are cleaved by a restriction endonuclease from Haemophilus aegyptius. J Biol Chem, 252:7300-7306. 1977. [PubMed]

Fuchs R., Blakesley R. Guide to the use of type II restriction endonucleases. Methods Enzymol, 100:3-38. 1983. [PubMed]

Thompson J.A., Blakesley R.W., Doran K., Hough C.J., Wells R.D. Purification of nucleic acids by RPC-5 ANALOG chromatography: peristaltic and gravity-flow applications. Methods Enzymol, 100:368-399. 1983. [PubMed]

Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F. Genetic organization of the KpnI restriction-modification system. Nucleic Acids Res, 19(23):6505-6509. 1991. [PubMed]

Wang G., Blakesley R.W., Berg D.E., Berg C.M. pDUAL: a transposon-based cosmid cloning vector for generating nested deletions and DNA sequencing templates in vivo. Proc Natl Acad Sci U S A, 90(16): 7874-7878. 1993. [PubMed]

Blakesley R.W. Cycle sequencing. Methods Mol Biol, 167:101-112. 2001. [PubMed]

Shevchenko Y., Bouffard G.G., Butterfield Y.S., Blakesley R.W., Hartley J.L., Young A.C., Marra M.A., Jones S.J., Touchman J.W., Green E.D. Systematic sequencing of cDNA clones using the transposon Tn5. Nucleic Acids Res, 30(11):2469-77. 2002. [PubMed]

Thomas J.W., Touchman J.W., Blakesley R.W., Bouffard G.G., Beckstrom-Sternberg S.M., Margulies E.H., Blanchette M., Siepel A.C., Thomas P.J., McDowell J.C., Maskeri B., Hansen N.F., Schwartz M.S., Weber R.J., Kent W.J., Karolchik D., Bruen T.C., Bevan R., Cutler D.J., Schwartz S., Elnitski L., Idol J.R., Prasad A.B., Lee-Lin S-Q., Maduro V.V.B., Summers T.J., Portnoy M.E., Dietrich N.L., Akhter N., Ayele K., Benjamin B., Cariaga K., Brinkley C.P., Brooks S.Y., Granite S., Guan X., Gupta J., Haghighi P., Ho S-L., Huang M.C., Karlins E., Laric P.L., Legaspi R., Lim M.J., Maduro Q.L., Masiello C.A., Mastrian S.D., McCloskey J.C., Pearson R., Stantripop S., Tiongson E.E., Tran J.T., Tsurgeon C., Vogt J.L., Walker M.A., Wetherby K.D., Wiggins L.S., Young A.C., Zhang L-H., Osoegawa K., Zhu B., Zhao B., Shu C.L., De Jong P.J., Lawrence C.E., Smit A.F., Chakravarti A., Haussler C., Green P., Miller W., and Green E.D. Comparative analyses of multi-species sequences from targeted genomic regions. Nature, 424(6950):788-793. 2003. [PubMed]

Blakesley, R.W., Hansen, N.F., Mullikin, J.C., Thomas, P.J., McDowell, J.C., Maskeri, B., Young A.C., Benjaminn, B., Brooks, S.Y., Coleman, B.I., Gupta, J., Ho, S-L., Karlins, E.M., Maduro, Q.L., Stantripop, S., Tsurgeon, C., Vogt, J.L., Walker, M.A., Masiello, C.A., Gua, X., NISC Comparative Sequencing Program, Bouffard, G.G., and Green, E.D. An intermediate grade of finished genomic sequence suitable for comparative analysis. Genome Research. 2004. [PubMed]

Margulies E.H., Cooper, G.M., Asimenos, G., Thomas, D.J., Dewey, C.N., Siepel, A., Birney, E., Keefe, D., Schwartz, A.S., Hou, M., Taylor, J., Nikolaev, S., Montoya-Burgos, J.I., Löytynoja, A., Whelan, S., Pardi, F., Massingham, T., Brown, J.B., Bickel, P., Holmes, I., Mullikin, J.C., Ureta-Vidal, A., Paten, B., Stone, E.A., Rosenbloom, K.R., Kent, W.J., Bouffard, G.G., Guan, X., Hansen, N.F., Idol, J.R., Maduro, V.V., Maskeri, B., McDowell, J.C., Park, M., Thomas, P.J., Young, A.C., Blakesley, R.W., Muzny, D.M., Sodergren, E., Wheeler, D.A., Worley, K.C., Jiang, H., Weinstock, G.M., Gibbs, R.A., Graves, T., Fulton, R., Mardis, E.R., Wilson, R.K., Clamp, M., Cuff, J., Gnerre, S., Jaffe, D.B., Chang, J.L., Lindblad-Toh, K., Lander, E.S., Hinrichs, A., Trumbower, H., Clawson, H., Zweig, A., Kuhn, R.M., Barber, G., Harte, R., Karolchik, D., Field, M.A., Moore, R.A., Matthewson, C.A., Schein, J.E., Marra, M.A., Antonarakis, S.E., Batzoglou, S., Goldman, N., Hardison, R., Haussler D., Miller, W., Pachter, L., Green, E.D., Sidow, A. Analyses of deep mammalian sequence alignments and constraint predictions for 1% of the human genome. Genome Res, 17(6):760-74. 2007. [PubMed]

The ENCODE Project Consortium. Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. Nature, 447: 799-816. 2007. [PubMed]

Grice EA, Kong HH, Conlan S, Deming CB, Davis J, Young AC; NISC Comparative Sequencing Program, Bouffard GG, Blakesley RW, Murray PR, Green ED, Turner ML, Segre JA. Topographical and Temporal Diversity of the Human Skin Microbiome. Science, 324,1190-1192. 2009. [PubMed]

Biesecker LG, Mullikin JC, Facio FM, Turner C, Cherukuri PF, Blakesley RW, Bouffard GG, Chines PS, Cruz P, Hansen NF, Teer JK, Maskeri B, Young AC; NISC Comparative Sequencing Program, Manolio TA, Wilson AF, Finkel T, Hwang P, Arai A, Remaley AT, Sachdev V, Shamburek R, Cannon RO, Green ED. The ClinSeq Project: Piloting Large-scale Genome Sequencing for Research in Genomic Medicine. Genome Res, 19, 1665-1674. 2009. [PubMed]

Blakesley RW, Hansen NF, Gupta J, McDowell JC, Maskeri B, Barnabas BB, Brooks SY, Coleman H, Haghighi P, Ho S-L, Schandler K, Stantripop S, Vogt JL, Thomas PJ; NISC Comparative Sequencing Program, Bouffard GG, Green ED. Effort Required to Finish Shotgun-generated Genome Sequences Differs Significantly Among Vertebrates. BMC Genomics, 11:21. 2010. [PubMed]

Davis E.E., Zhang Q., Liu Q., Diplas B.H., Davey L.M., Hartley J., Stoetzel C., Szymanska K., Ramaswami G., Logan C.V., Muzny D.M., Young A.C., Wheeler D.A., Cruz P., Morgan M., Lewis L.R., Cherukuri P., Maskeri B., Hansen N.F., Mullikin J.C., Blakesley R.W., Bouffard G.G.; NISC Comparative Sequencing Program, Gyapay G., Rieger S., Tönshoff B., Kern I., Soliman N.A., Neuhaus T.J., Swoboda K.J., Kayserili H., Gallagher T.E., Lewis R.A., Bergmann C., Otto E.A., Saunier S., Scambler P.J., Beales P.L., Gleeson J.G., Maher E.R., Attié-Bitach T., Dollfus H., Johnson C.A., Green E.D., Gibbs R.A., Hildebrandt F., Pierce E.A., Katsanis N. (2011) TTC21B contributes both causal and modifying alleles across the ciliopathy spectrum. Nat Genet, 43:189-196; and corrigendum Nat Genet, 43:499. 2011. [PubMed]
Conlan S., Mijares L.A., Comp Seq Program N, Becker J., Blakesley R.W., Bouffard G.G., Brooks S., Coleman H.L., Gupta J., Gurson N., Park M., Schmidt B., Thomas P.J., Young A., Otto M., Kong H.H., Murray P.R., Segre J.A. Staphylococcus epidermidis pan-genome sequence analysis reveals diversity of skin commensal and hospital infection-associated isolates. Genome Biol, 13:R64. 2012. [PubMed]

Schnitzler C.E., Pang K., Powers M.L., Reitzel A.M., Ryan J.F., Simmons D., Tada T., Park M., Gupta J., Brooks S.Y., Blakesley R.W., Yokoyama S., Haddock S.H., Martindale M.Q., Baxevanis A.D. Genomic organization, evolution, and expression of photoprotein and opsin genes in Mnemiopsis leidyi: A new view of ctenophore photocytes. BMC Biol, 10:107. 2012. [PubMed]

Zhu J., Ofek G., Yang Y., Zhang B., Louder M.K., Lu G., McKee K., Pancera M., Skinner J., Zhang Z., Parks R., Eudailey J., Lloyd K.E., Blinn J., Alam S.M., Haynes B.F., Simek M., Burton D.R., Koff W.C.; NISC Comparative Sequencing Program, Mullikin J.C., Mascola J.R., Shapiro L., Kwong P.D. Mining the antibodyome for HIV-1-neutralizing antibodies with next-generation sequencing and phylogenetic pairing of heavy/light chains. Proc Natl Acad Sci USA, 110:6470-6475. 2013. [PubMed]
Bentley A.R., Chen G., Shriner D., Doumatey A.P., Zhou J., Huang H., Mullikin J.C., Blakesley R.W., Hansen N.F., Bouffard G.G., Cherukuri P.F., Maskeri B., Young A.C., Adeyemo A., Rotimi C.N. Gene-based sequencing identifies lipid-influencing variants with ethnicity-specific effects in african americans. PLoS Genet, 10:e1004190. 2014. [PubMed

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Last Updated: July 10, 2014