James Inglese, Ph.D.

Adjunct Investigator, Cancer Genetics Branch, National Human Genome Research Institute
Group Leader, Assay Development and Screening Technology Laboratory, National Center for Advancing Translational Sciences

Selected Publications

Galivan J, Inglese J, McGuire JJ, Nimac Z, Coward JK. gamma-fluoromethotrexate: synthesis and biological activity of a potent inhibitor of dihydrofolate reductase with greatly diminished ability to form poly-gamma-glutamates. Proc Nat Acad Sci 82:2598-2602, 1985. [PubMed]

Inglese J, Blatchly RA, Benkovic SJ. A multisubstrate adduct inhibitor of a purine biosynthetic enzyme with a picomolar dissociation constant. J Med Chem, 32:937-940. 1989. [PubMed]

Inglese J. Expanding the high throughput screening paradigm. Drug Discov Today, 7 Suppl 18:S105-106. 2002. [PubMed]

Inglese J, Auld DS, Jadhav A, Johnson RL, Simeonov A, Yasgar A, Zheng W, Austin CP. Quantitative high-throughput screening: a titration-based approach that efficiently identifies biological activities in large chemical libraries. Proc Natl Acad Sci USA, 103:11473-11478. 2006. [PubMed]

Auld DS, Johnson RL, Zhang Y, Veith H, Jadhav A, Yasgar A, Simeonov A, Zheng W, Martinez ED,... Inglese J. Fluorescent protein-based cellular assays analyzed by laser scanning microplate cytometry in 1536-well plate format. Methods Enzymol, 414:566-589. 2006. [PubMed]

Davis ER, Zhang Y, Southall N, Staudt LM, Austin CP, Inglese J, Auld D. A cell-based assay for IkappaBalpha stabilization using a two-color dual luciferase-based sensor. Assay Drug Dev Technol. 5:85-104, 2007. [PubMed]

Inglese J, Shamu CE, Guy RK. Reporting data from high throughput screening of small molecule libraries. Nat Chem Biol. 3:438-441, 2007. [PubMed]

Inglese J, Johnson RL, Simeonov A, Xia M, Zheng W, Austin CP, Auld DS. High-throughput screening assays for the identification of chemical probes. Nat Chem Biol, 3:466-479. 2007. [PubMed]

Sayed AA, Simeonov A, Thomas CJ, Inglese J, Austin CP, Williams DL. Identification of oxadiazoles as new drug leads for the control of schistosomiasis. Nat Med, 14:407-412. 2008. [PubMed]

Auld DS, Thorne N, Nguyen DT, Inglese J. A specific mechanism for nonspecific activation in reporter-gene assays. ACS Chem Biol, 3:463-470. 2008. [PubMed]

Inglese J, Auld DS. Application of high throughput screening (HTS) techniques: overview of applications in Chemical Biology. In: Begley TP (Ed), Wiley Encyclopedia of Chemical Biology, Vol. 2. Hoboken: John Wiley & Sons, pp. 260-274. 2009.

Yuan J, Johnson RL, Huang R, Wichterman J, Jiang H, Hayton K, Fidock DA, Wellems TE, Inglese J,... Su XZ. Screening and genetic mapping targets of differential chemical-response phenotypes in Plasmodium falciparum. Nat Chem Biol, 5:765-771. 2009. [PubMed]

Auld DS, Lovell S, Thorne N, Lea WA, Maloney DJ, Shen M, Rai G, Battaile KP, Thomas CJ,... Inglese J. Molecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124. Proc Natl Acad Sci USA, 107:4878-4883. 2010. [PubMed]

Thorne N, Inglese J, Auld DS. Illuminating insights into firefly luciferase and other bioluminescent report­ers used in chemical biology. Chem Biol, 17:646-657. 2010. [PubMed]

Thorne N, Auld DS, Inglese J. Apparent activity in high-throughput screening: origins of compound-dependent assay interference. Curr Opin Chem Biol, 14:315-324. 2010. [PubMed]

Yuan, J., Cheng, K. C.-C., Johnson, R.L., Huang, R., Pattaradilokrat, S., Liu, A., Guha, R., Fidock, D., Inglese, J., Wellems, T.E., Austin, C.P., & Su, x.-Z. Chemical genomic profiling for antimalarial therapies, response signatures and molecular targets, Science, 333:724-729. 2011. [PubMed]

Cruz, P.G., Auld, D.S., Schultz, P.J., Lovell, S., Battaile, K.P., MacArthur, R., Shen, M., Tamayo, G., Inglese, J., Sherman, D.H. Titration-based screening for evaluation of natural product extracts: identification of an aspulvinone family of luciferase inhibitors. Chem Biol, 18:1442-1452. 2011. [PubMed]

Top of page

Last Updated: January 20, 2012