Online Education Kit: Separating the Sequencing Products: Transcript

National Human Genome Research Institute

National Institutes of Health
U.S. Department of Health and Human Services


Separating the Sequencing Products: Transcript

The DNA molecules produced during the sequencing reaction are separated from each other by a process called electrophoresis. DNA molecules are negatively charged. The sequencing machine sets up an electric field; all the DNA moves through a porous gel toward the positive electrode. The gel acts like a sieve; shorter DNA fragments move more quickly through the holes of the gel than do larger DNA fragments.


Last Reviewed: March 29, 2012