Blakesley Group

Robert W. Blakesley, Ph.D.

Director, Sequencing Group, NIH Intramural Sequencing Center []
Associate Investigator, NIH Intramural Sequencing Center (NISC)

Selected Publications

Boezi J.A., Lee L.F., Blakesley R.W., Koening M., and Towle H.C. Marek's disease herpesvirus - induced DNA polymerase. J. Virology, 14:1209-1219. 1974. [PubMed]
Blakesley R.W. and Boezi J.A. A kinetic and structural characterization of adenosine 5'-triphosphate:ribonucleic acid adenylyltransferase from Pseudomonas putida. Biochem Biophys Acta, 414:133-145. 1975. [PubMed]
Blakesley R.W., Dodgson J.B., Nes I.F. and Wells, R.D. Duplex regions in single-stranded FX174 are cleaved by a restriction endonuclease from Haemophilus aegyptius. J Biol Chem, 252:7300-7306. 1977. [PubMed]

Fuchs R., Blakesley R. Guide to the use of type II restriction endonucleases. Methods Enzymol, 100:3-38. 1983. [PubMed]

Thompson J.A., Blakesley R.W., Doran K., Hough C.J., Wells R.D. Purification of nucleic acids by RPC-5 ANALOG chromatography: peristaltic and gravity-flow applications. Methods Enzymol, 100:368-399. 1983. [PubMed]

Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F. Genetic organization of the KpnI restriction-modification system. Nucleic Acids Res, 19(23):6505-6509. 1991. [PubMed]

Wang G., Blakesley R.W., Berg D.E., Berg C.M. pDUAL: a transposon-based cosmid cloning vector for generating nested deletions and DNA sequencing templates in vivo. Proc Natl Acad Sci U S A, 90(16): 7874-7878. 1993. [PubMed]

Blakesley R.W. Cycle sequencing. Methods Mol Biol, 167:101-112. 2001. [PubMed]

Shevchenko Y., Bouffard G.G., Butterfield Y.S., Blakesley R.W., Hartley J.L., Young A.C., Marra M.A., Jones S.J., Touchman J.W., Green E.D. Systematic sequencing of cDNA clones using the transposon Tn5. Nucleic Acids Res, 30(11):2469-77. 2002. [PubMed]

Thomas J.W., Touchman J.W., Blakesley R.W., Bouffard G.G., Beckstrom-Sternberg S.M., Margulies E.H., Blanchette M., Siepel A.C., Thomas P.J., McDowell J.C., Maskeri B., Hansen N.F., Schwartz M.S., Weber R.J., Kent W.J., Karolchik D., Bruen T.C., Bevan R., Cutler D.J., Schwartz S., Elnitski L., Idol J.R., Prasad A.B., Lee-Lin S-Q., Maduro V.V.B., Summers T.J., Portnoy M.E., Dietrich N.L., Akhter N., Ayele K., Benjamin B., Cariaga K., Brinkley C.P., Brooks S.Y., Granite S., Guan X., Gupta J., Haghighi P., Ho S-L., Huang M.C., Karlins E., Laric P.L., Legaspi R., Lim M.J., Maduro Q.L., Masiello C.A., Mastrian S.D., McCloskey J.C., Pearson R., Stantripop S., Tiongson E.E., Tran J.T., Tsurgeon C., Vogt J.L., Walker M.A., Wetherby K.D., Wiggins L.S., Young A.C., Zhang L-H., Osoegawa K., Zhu B., Zhao B., Shu C.L., De Jong P.J., Lawrence C.E., Smit A.F., Chakravarti A., Haussler C., Green P., Miller W., and Green E.D. Comparative analyses of multi-species sequences from targeted genomic regions. Nature, 424(6950):788-793. 2003. [PubMed]

Blakesley, R.W., Hansen, N.F., Mullikin, J.C., Thomas, P.J., McDowell, J.C., Maskeri, B., Young A.C., Benjaminn, B., Brooks, S.Y., Coleman, B.I., Gupta, J., Ho, S-L., Karlins, E.M., Maduro, Q.L., Stantripop, S., Tsurgeon, C., Vogt, J.L., Walker, M.A., Masiello, C.A., Gua, X., NISC Comparative Sequencing Program, Bouffard, G.G., and Green, E.D. An intermediate grade of finished genomic sequence suitable for comparative analysis. Genome Research. 2004. [PubMed]

Margulies E.H., Cooper, G.M., Asimenos, G., Thomas, D.J., Dewey, C.N., Siepel, A., Birney, E., Keefe, D., Schwartz, A.S., Hou, M., Taylor, J., Nikolaev, S., Montoya-Burgos, J.I., Löytynoja, A., Whelan, S., Pardi, F., Massingham, T., Brown, J.B., Bickel, P., Holmes, I., Mullikin, J.C., Ureta-Vidal, A., Paten, B., Stone, E.A., Rosenbloom, K.R., Kent, W.J., Bouffard, G.G., Guan, X., Hansen, N.F., Idol, J.R., Maduro, V.V., Maskeri, B., McDowell, J.C., Park, M., Thomas, P.J., Young, A.C., Blakesley, R.W., Muzny, D.M., Sodergren, E., Wheeler, D.A., Worley, K.C., Jiang, H., Weinstock, G.M., Gibbs, R.A., Graves, T., Fulton, R., Mardis, E.R., Wilson, R.K., Clamp, M., Cuff, J., Gnerre, S., Jaffe, D.B., Chang, J.L., Lindblad-Toh, K., Lander, E.S., Hinrichs, A., Trumbower, H., Clawson, H., Zweig, A., Kuhn, R.M., Barber, G., Harte, R., Karolchik, D., Field, M.A., Moore, R.A., Matthewson, C.A., Schein, J.E., Marra, M.A., Antonarakis, S.E., Batzoglou, S., Goldman, N., Hardison, R., Haussler D., Miller, W., Pachter, L., Green, E.D., Sidow, A. Analyses of deep mammalian sequence alignments and constraint predictions for 1% of the human genome. Genome Res, 17(6):760-74. 2007. [PubMed]

The ENCODE Project Consortium. Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. Nature, 447: 799-816. 2007. [PubMed]

Grice EA, Kong HH, Conlan S, Deming CB, Davis J, Young AC; NISC Comparative Sequencing Program, Bouffard GG, Blakesley RW, Murray PR, Green ED, Turner ML, Segre JA. Topographical and Temporal Diversity of the Human Skin Microbiome. Science, 324,1190-1192. 2009. [PubMed]

Biesecker LG, Mullikin JC, Facio FM, Turner C, Cherukuri PF, Blakesley RW, Bouffard GG, Chines PS, Cruz P, Hansen NF, Teer JK, Maskeri B, Young AC; NISC Comparative Sequencing Program, Manolio TA, Wilson AF, Finkel T, Hwang P, Arai A, Remaley AT, Sachdev V, Shamburek R, Cannon RO, Green ED. The ClinSeq Project: Piloting Large-scale Genome Sequencing for Research in Genomic Medicine. Genome Res, 19, 1665-1674. 2009. [PubMed]

Blakesley RW, Hansen NF, Gupta J, McDowell JC, Maskeri B, Barnabas BB, Brooks SY, Coleman H, Haghighi P, Ho S-L, Schandler K, Stantripop S, Vogt JL, Thomas PJ; NISC Comparative Sequencing Program, Bouffard GG, Green ED. Effort Required to Finish Shotgun-generated Genome Sequences Differs Significantly Among Vertebrates. BMC Genomics, 11:21. 2010. [PubMed]

Conlan S., Mijares L.A., Comp Seq Program N, Becker J., Blakesley R.W., Bouffard G.G., Brooks S., Coleman H.L., Gupta J., Gurson N., Park M., Schmidt B., Thomas P.J., Young A., Otto M., Kong H.H., Murray P.R., Segre J.A. Staphylococcus epidermidis pan-genome sequence analysis reveals diversity of skin commensal and hospital infection-associated isolates. Genome Biol, 13:R64. 2012. [PubMed]

Schnitzler C.E., Pang K., Powers M.L., Reitzel A.M., Ryan J.F., Simmons D., Tada T., Park M., Gupta J., Brooks S.Y., Blakesley R.W., Yokoyama S., Haddock S.H., Martindale M.Q., Baxevanis A.D. Genomic organization, evolution, and expression of photoprotein and opsin genes in Mnemiopsis leidyi: A new view of ctenophore photocytes. BMC Biol, 10:107. 2012. [PubMed]

Ferreira Z., Hurle B., Andrés A.M., Kretzschmar W.W., Mullikin J.C., Cherukuri P.F., Cruz P., Gonder M.K., Stone A.C., Tishkoff S., Swanson W.J.; NISC Comparative Sequencing Program, Green E.D., Clark A.G., Seixas S. Sequence diversity of Pan troglodytes subspecies and the impact of WFDC6 selective constraints in reproductive immunity. Genome Evol Biol, 5:2512-2523. 2013. [PubMed]

Zhu J., Ofek G., Yang Y., Zhang B., Louder M.K., Lu G., McKee K., Pancera M., Skinner J., Zhang Z., Parks R., Eudailey J., Lloyd K.E., Blinn J., Alam S.M., Haynes B.F., Simek M., Burton D.R., Koff W.C.; NISC Comparative Sequencing Program, Mullikin J.C., Mascola J.R., Shapiro L., Kwong P.D. Mining the antibodyome for HIV-1-neutralizing antibodies with next-generation sequencing and phylogenetic pairing of heavy/light chains. Proc Natl Acad Sci USA, 110:6470-6475. 2013. [PubMed]
Bentley A.R., Chen G., Shriner D., Doumatey A.P., Zhou J., Huang H., Mullikin J.C., Blakesley R.W., Hansen N.F., Bouffard G.G., Cherukuri P.F., Maskeri B., Young A.C., Adeyemo A., Rotimi C.N. Gene-based sequencing identifies lipid-influencing variants with ethnicity-specific effects in african americans. PLoS Genet, 10:e1004190. 2014. [PubMed

Callicrate T, Dikow R, Thomas JW, Mullikin JC, Jarvis ED, Fleischer RC; NISC Comparative Sequencing Program. Genomic resources for the endangered Hawaiian honeycreepers. BMC Genomics, 15:1098. 2014. [PubMed]

Rudd ML, Mohamed H, Price JC, O'Hara AJ, Le Gallo M, Urick ME; NISC Comparative Sequencing Program, Cruz P, Zhang S, Hansen NF, Godwin AK, Sgroi DC, Wolfsberg TG, Mullikin JC, Merino MJ, Bell DW. Mutational analysis of the tyrosine kinome in serous and clear cell endometrial cancer uncovers rare somatic mutations in TNK2 and DDR1. BMC Cancer, 14:884. 2014. [PubMed]

Conlan S, Thomas PJ, Deming C, Park M, Lau AF, Dekker JP, Snitkin ES, Clark TA, Luong K, Song Y, Tsai YC, Boitano M, Dayal J, Brooks SY, Schmidt B, Young AC, Thomas JW, Bouffard GG, Blakesley RW; NISC Comparative Sequencing Program, Mullikin JC, Korlach J, Henderson DK, Frank KM, Palmore TN, Segre JA. Single-molecule sequencing to track plasmid diversity of hospital-associated carbapenemase-producing Enterobacteriaceae. Sci Transl Med, 6:254ra126. 2014. [PubMed]

Oh J, Byrd AL, Deming C, Conlan S; NISC Comparative Sequencing Program, Kong HH, Segre JA. Biogeography and individuality shape function in the human skin metagenome. Nature, 514:59-64. 2014. [PubMed]

Zhou T, Lynch RM, Chen L, Acharya P, Wu X, Doria-Rose NA, Joyce MG, Lingwood D, Soto C, Bailer RT, Ernandes MJ, Kong R, Longo NS, Louder MK, McKee K, O'Dell S, Schmidt SD, Tran L, Yang Z, Druz A, Luongo TS, Moquin S, Srivatsan S, Yang Y, Zhang B, Zheng A, Pancera M, Kirys T, Georgiev IS, Gindin T, Peng HP, Yang AS; NISC Comparative Sequencing Program, Mullikin JC, Gray MD, Stamatatos L, Burton DR, Koff WC, Cohen MS, Haynes BF, Casazza JP, Connors M, Corti D, Lanzavecchia A, Sattentau QJ, Weiss RA, West AP Jr, Bjorkman PJ, Scheid JF, Nussenzweig MC, Shapiro L, Mascola JR, Kwong PD. Structural repertoire of HIV-1-neutralizing antibodies targeting the CD4 supersite in 14 donors. Cell, 161:1280-1292. 2015. [PubMed]

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Last Updated: August 19, 2015