Online Education Kit: Reading the Sequencing Products: Transcript

National Human Genome Research Institute

National Institutes of Health
U.S. Department of Health and Human Services


Reading the Sequencing Products: Transcript

As each DNA fragment reaches the end of the gel, a laser excites its fluorescent dye. A camera detects the color of the emitted light and passes that information to a computer. One by one, the machine records the colors of the DNA fragments that pass through the gel.

A single sequencing reaction can reveal the order of several hundred DNA bases.


Last Reviewed: March 29, 2012