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2019 Advanced Genomic Technology Development Meeting

Event Details

The National Human Genome Research Institute (NHGRI) hosted the Advanced Genomic Technology Development Meeting at Northeastern University in Boston, Massachusetts, on May 29 - 31, 2019. The purpose of the meeting was to provide an open forum on key scientific and technical challenges to advancing a broad set of genomic technologies including those for nucleic acid sequencing.  The meeting brought together NHGRI Genomic Technology Program grantees and other scientists and engineers who had insights that would help to achieve the goal of sequencing human genomes at high quality for much less than $1,000, and develop a broad suite of new genomic technologies.


Northeastern University
East Village 17th Floor Event Space
360 Huntington Avenue
Boston, Massachusetts 02115


  • Wednesday, May 29, 2019

  • 7:30 a.m. Breakfast, Registration and Poster Setup
  • 8:30 a.m. Welcome
    Mike Smith and Carolyn Hutter (NHGRI)
  • Genome Tech Awards
    Session Chair: Lisa Chadwick (NHGRI)
  • 8:50 a.m. Single-cell and single-molecule detection of epigenetic modifications
    Bradley Berstein
  • 9:07 a.m. Imaging and sequencing the epigenome in situ
    Feng Zhang
  • 9:24 a.m. Enzyme-less DNA base discrimination using solid-state nanopores with high-frequency integrated detection electronics
    Jason Buenrostro
  • 9:41 a.m. Spatio-genetic imaging with DNA microscopy (Regev)
    Joshua Weinstein
  • 9:58 a.m. Surveying transcription factors for pioneer binding specificity to nucleosomal DNA (Bulyk)
    Meni Wanunu
  • 10:15 a.m. BREAK
  • 10:45 a.m. First Discussion
    Stirling Churchman, Joseph Ecker, Chongyuan Luo, Karine Choquet
  • 11:45 a.m. Lightning Poster Preview: 
    Feldman (40), Carlson (pg. 39), Craig (28), Dell (30), Drndic (18), Hao (36), Kock (15), Le (41), Mulroney (24), Noakes (29), Pavlenok (19), Payne (12), Poodari (25), Razaghi (21), Sadowski (22), Singh (42), Stephenson (37), Tobar (32), Vo (26) & Yu (34) 

    Chair: Meni Wanunu
  • 12:10 p.m. Lunch
  • Poster Session
    New Sequencing Tech Awards
    Session Chair: Jacob Buchheim
  • 2:00 p.m. DNA Sequencing with 2D FET-nanopore devices
    Marja Dmdic
  • 2:10 p.m. Single-chain MspA for nanopore sequencing DNA 
    Michael Niederweis
  • 2:20 p.m. Using nanopore sequencing to interrogate the genome and epigenome
    Winston Timp
  • 2:30 p.m. Group Photo and Break
  • Genome & Seqnecing Tech Awards
    Session Chair: Andrew Laszlo
  • 3:15 p.m. Nanopore sequencing of human RNA: Baseline performance and optimization strategies
    Mark Akeson
  • 3:32 p.m. Towards high-accuracy nanopore sequencing
    Jens Gundlach
  • 3:49 p.m. Computational design engine for accurate and efficient sequencing of DNA and RNA
    Murugappan Muthukumar
  • 4:06 p.m. Improving read length, accuracy, and availability of single-molecule DNA sequencing
    Henk Postma
  • 4:23 p.m. Ultra-high-accuracy nanopore target-sequencing for innovations in HIV diagnosis and surveillance
    Sanggu Kim
  • 4:40 p.m. Simultaneous analysis of transcription factor binding and mRNA expression in single cells
    Rob Mitra
  • 4:57 p.m. Highly multimodal measurements of single cells
    Peter Smibert
  • 5:14 p.m. Pooled optical screening
    Paul Blainey
  • 5:31 p.m. Northeastern Reception


  • Thursday, May 30, 2019

  • 7:30 a.m. Breakfast
  • 8:25 a.m. Introduction 
    Mike Smith (NHGRI)
  • Sequencing Tech Awards
    Session Chair: Henry Brinkerhoff (Gundlach)
  • 8:30 a.m. Porous Zero-Mode Waveguides for pg-level RNA and DNA Sequencing
    Meni Wanunu
  • 8:47 a.m. Nanopore-based applications for labelled RNAs
    Direct nanopore detection of modified RNA to probe structure and dynamics
  • 9:04 a.m. A general LC/MS-based RNA sequencing method for direct analysis of multiplebase modifications in synthetic and biological RNAs at single-base resolution
    Shenglong Zhang
  • 9:21 a.m. DNA sequencing with two-dimensional nanopores and multiplexed CMOS electronics
    Steve Tung
  • 9:38 a.m. Standards in genomic technology: what do we need over the next 5 years
    Stuart Lindsay
  • 9:38 a.m. BREAK
  • 10:30 a.m. Second Discussion: Grantsmanship
    Moderators: Mary Jelinek, Prashant Mali, Fei Chen
  • 11:30 a.m. Lightning Poster Preview: Briggs (pg. 75), Charron (76), Dapprich (77), Kanavarioti (78), Liu (79), Maryanski (80), McDonald (58), Niedzwiecki (82), Perez (83), Trotta (84), Waugh (85), Zhang (46) & Zhang (50)

    Chair: Meni Wanunu
  • 12:00 p.m. Lunch
  • New Genome & Sequencing Awards
    Session Chair: Jyoti Mahalik
  • 2:00 p.m. A transposase system for integrative ChIP-exo and ATAC-seq analysis
    Julia Zeitlinger
  • 2:10 a.m. High-throughput, multiplexed methods for the epigenomic characterization of human disease states
    Mary Anne Jelinek
  • 2:20 p.m. Direct measurement of m1A methylated RNA using the Tempo-Seq targeted gene expression assay
    Bruce Seligmann
  • 2:30 p.m. Exonuclease enabled DNA sequencing
    Eric Ervin
  • 2:40 p.m. Immobilized exonuclease-mediated single-molecule sequencing using polymerderived nanosensor arrays
    Aaron Nagel
  • 2:50 p.m. Super-resolution sequencing
    Kalim Mir
  • 3:00 p.m. Mark Pratt
    Novel high throughput sequencing platform
  • 3:10 p.m. Break
  • Genome and Sequencing Tech Awards
    Session Chair: Kian Hong Kock
  • 3:40 p.m. Toward single-molecule diagnostics with solid-state nanopores at bandwidths in excess of 10 MHz
    Kenneth Shepard
  • 3:57 p.m. Towards enabling improved human genome interpretation via a new biological model system
    Prashant Mali
  • 4:14 p.m. Multi-omic analysis of single cells
    Chongyuan Luo
  • 4:31 p.m. Drop-BS: High-throughput single-cell bisulfite sequencing on a microfluidic droplet platform
    Chang Lu
  • 4:48 p.m. Nanofluidic platforms for single-molecule characterization of genomic DNA
    Laurent Menard
  • 5:05 p.m. Break before Student & Post-doc Receptions
  • 5:40 p.m. Student & Post-doc Session (with light dinner):
    Career Transitions and Challenges

    Kristin Gabriel, Chongyuan Luo, Ning Zhang, Logan Mulroney, Avtar Singh, Joshua Weinstein


  • Friday, May 31, 2019

  • 7:30 a.m. Breakfast
  • 8:00 a.m. Open Meeting Welcome
    Carolyn Hutter and Mike Smith (NHGRI)
  • Plenary Session
    Session Chair: David Feldman
  • 8:45 a.m. Telomere-to-telomere assembly of a complete human X chromosome
    Karen Miga
  • 9:10 a.m. Single-molecule electronic measurements of DNA polymerases
    Philip Collins
  • 9:35 a.m. An ultra-processive RT for monitoring sequence and structural variation in long, structured RNA templates
    Anna Pyle
  • 10:00 a.m. Slide-Seq: A Scalable Technology for Measuring Genome-Wide Expression at High Spatial Resolution
    Fei Chen
  • 10:25 a.m. Break
  • 11:00 a.m. Genomic Medicine: Technology Today and Challenges for Tomorrow
    Anastasia Wise
  • 12:00 p.m. Poster Session and Lunch
  • 1:00 p.m. NHGRI Strategic Planning Session

    Background & Introduction: Carolyn Hutter, NHGRI (20 mins)
    Nucleic Acid Technologies: Jens Gundlach, John Nelson, Winston Timp, Anna Pyle (20 mins)
    Chromatin Structure & Gene Regulation: Jason Buenrostro, Julia Zeitlinger, Joshua Weinstein, Mary Jelinek (20 mins)
    Functional Genomics: Rob Mitra, Paul Blainey, Joseph Ecker (20 mins)
    Wrap-up: Mike Smith, NHGRI (10 mins)
  • 3:00 p.m. Break
  • Genome & Sequencing Tech Awards
    Session Chair: Fatemeh Farhangdoust
  • 3:30 p.m. Exploring RNA splicing regulation with direct sequencing of nascent RNA
    Karine Choquet
  • 3:45 p.m. Advancing solid-state nanopores for nucleic acid analysis: tackling challenges in fabrication, capture and passage
    Kyle Briggs
  • 4:00 p.m. Arima-HiC: A platform technology accelerating the understanding of genome sequence and structure
    Siddarth Selvaraj
  • 4:15 p.m. Single-molecule activity of TAQ polymerase at 72 °C
    Mackenzie Turvey
  • 4:30 p.m. Adjourn

Last updated: August 1, 2019