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2021 Advanced Genomic Technology Development Meeting

Event Details

The National Human Genome Research Institute (NHGRI) hosted the virtual Advanced Genomic Technology Development Meeting, on May 25-27, 2021. The purpose of the virtual meeting series is to provide an open forum to discuss key scientific and technical challenges faced with advanced sequencing, genomic, and synthetic nucleic acid technologies. The meeting will bring together NHGRI Genomic Technology Program grantees, other scientists, and engineers who have insights that could synergistically help to develop a broad suite of novel methods. Activities are designed to update fellow grantees and NHGRI about progress in the field and specific funded projects, facilitate collaborations, and provide a forum that effectively serves the entire set of participants.



Virtual Meeting Agenda

  • Tuesday, May 25, 2021

  • 11:00 a.m. Introduction
  • Grantee Talks
    Moderator: Miten Jain (PI: Mark Akeson)
  • Nanopore Sequencing Innovation
  • 11:05 a.m. Towards high accuracy nanopore sequencing
    Jens Gundlach
  • 11:25 a.m. Computational design engine for accurate and efficient sequencing of DNA and RNA
    Murugappan Muthukumar
  • 11:45 a.m. Chemically tuned silicon nitride nanopores for nucleic acid sequencing
    Jason Dwyer
  • 12:05 p.m. Single-cell direct RNA sequencing using electrical zero-mode waveguides and engineered reverse transcriptases
    Meni Wanunu
  • 12:25 p.m. Capturing motor/nucleotide complexes onto solid-state structures
    Sean German
  • 12:45 p.m. Break
  • Grantee Talk
    Moderator: Rebecca Carlson (PI: Paul Blainey)
  • Base Modification Sequencing
  • 1:15 p.m. DROP-BS: High-throughput single-cell bisulfite sequencing on a microfluidic droplet platform
    Qiang Zhang (Lu lab)
  • Trainee Talks
  • 1:35 p.m. Massively multiplexed, whole-embryo developmental genetics at single-cell resolution
    Lauren Saunders
  • 1:45 p.m. Diverse enzymatic activities mediate antiviral immunity in prokaryotes
    Linyi Gao
  • 1:55 p.m. Ion fountain nanopore readers for high-resolution DNA and RNA sequencing
    Mehrnaz Mojtabavi
  • 2:05 p.m. NHGRI Coordinating Center Update
  • 2:35 p.m. Break
  • Grantee Talks
    Moderator: Sohini Mukherjee (PI: Stuart Lindsay)
  • Enzyme Sequencing Innivation
  • 2:45 p.m. A polymerase with electrical connections and strand displacement activity
    Stuart Lindsay
  • 3:05 p.m. Engineering 3DPol for RNA sequencing by protein conductance
    Ya-Ming Hou
  • 3:25 p.m. Breakout Special Topics Session
  • Single cell analysis
    Chairs: Jay Shendure, Fei Chen

    Chairs: Joe Ecker, Julia Zeitlinger

    Editing for Genomics
    Chairs: Feng Zhang, Paul Blainey

    Enzymes as Tools
    Chairs: Ya-Ming Hou, Phil Collins

    Sequencing Innovation
    Chairs: Meni Wanunu, Winston Timp
  • 4:10 p.m. End of Day 1


  • Wednesday, May 26, 2021

  • Grantee Talks
    Moderator: Florence Chardon (PIs: Shendure/Trapnell)
  • Base Modification Sequencing
  • 11:00 a.m. Direct nanopore sequencing of individual full length tRNA strands
    Mark Akeson
  • 11:20 a.m. Nanopore sequencing to interrogate the genome, epigenome, and transcriptome
    Winston Timp
  • 11:40 a.m. Direct Determination of Multiple Specific Forms of DNA Chemical Modifications
    Gang Fang
  • 12:00 p.m. Enzymatic approaches to profiling epigenetic DNA modifications
    Rahul Kohli
  • 12:20 p.m. Targeted sequencing assay of RNA methylation
    Bruce Seligmann
  • 12:40 p.m. Break
  • Grantee Talks
    Moderator: Wei Yang (Shendure/Trapnell)
  • Nanopore Sequencing Innovation
  • 1:10 p.m. Single-molecule electronic nucleic acid sequencing-by- synthesis using tagged nucleotides and nanopore constructs
    Jingyue Ju
  • Multiomics
  • 1:30 p.m. A transposase system for integrative ChIP-EXO and ATAC-seq analysis
    Sabrina Krueger (Zeitlinger lab)
  • 1:50 p.m. High throughput, multiplexed epigenomic profiling of primary tissue for molecular interrogation of clinical disease states
    Mary Anne Jelinek
  • Pooled Screening
  • A Genome-Wide Optical Pooled Screen Identifies Novel Regulators of IRF3 Translocation
    Rebecca Carlson (Blainey lab)
  • 2:30 p.m. Break
  • Trainee Talks
    Moderator: Sanary Srivatsan (PIs: Shendure/Trapnell)
  • 2:45 p.m. Approaching in vivo optical pooled CRISPR screens utilizing in situ sequencing readout from tissue sections
    Michael Leiken
  • 2:55 p.m. Rank-based statistical methods for the identification of subcellular RNA localization patterns from transcriptome-wide multiplexed fish datasets
    Rob Bierman
  • 3:05 p.m. SCITO-Seq: Single-cell Combinatorial Indexed Cytometry Sequencing
    Byungjin Hwang (Ye lab)
  • 3:15 p.m. Trainee Session - Forming cross-disciplinary collaborations
    Moderator: Sanjay Srivatsan
    Panelists: Cole Trapnell, Ya-Ming Hou, Jason Dwyer
  • 4:00 p.m. End Day 2


  • Thursday, May 27, 2021

  •  11:00 a.m. Breakout Session Report Back
  • Trainee Talks
    Moderator: Byungiin Hwang (PI: Jimmie Ye)
  • 11:30 a.m. Single cell RNA-sequencing of 1.2 million cells reveals cellular genetic correlates of systemic lupus erythematosus
    M. Grace Gordon
  • 11:40 a.m. Identifying regulators of synaptogenesis with optical pooled screens
    Anna Le
  • 11:50 a.m. Characterizing solid-state nanopores
    Brian Sheetz
  • Grantee Talks
  • Editing Technologies
  • 12:00 p.m. Programmable RNA editing via ADARs
    Prashant Mali
  • 12:20 p.m. Exploration of Biological Diversity to Discover Novel Molecular Technologies
    Feng Zhang
  • 12:40 p.m. Break
  • Grantee Talks
    Moderator Anna Le (PI: Paul Blainey)
  • Chromatin
  • 1:00 p.m. Surveying sequence and histone influences of nucleosomes on transcription factor pioneer binding
    Luca Mariani (Bulyk lab)
  • Single Cell Multi-Omics
  • 1:20 p.m. A single cell spatial genomics platform for multi-modal characterization of tissue organization
    Fei Chen
  • 1:40 p.m. Single cell multi-tissue multi-omic profiling of 3D genome organization and DNA methylation
    Jingtian Zhou (Ecker lab)
  • 2:00 p.m. Versatile, exponentially scalable methods for single cell molecular profiling
    Sanjay Srivatsan (Shendure/Trapnell labs)
  • 2:20 p.m. Break
  • Grantee Talks
    Moderator: Chongyuan Luo (PI: Joseph Ecker)
  • Sequencing for Biology
  • 2:30 p.m. Genetic and epigenetic maps of endogenous human centromeres
    Karen Miga
  • 2:50 p.m. High-fidelity, portable Nanopore sequencing for RNA virus detection and surveillance
    Sanggu Kim
  • 3:10 p.m. Trainee Session - Navigating your career after your degree
    Moderator: Chongyuan Luo
    Panelists: Jo Yeakley, Gang Fang
  • 3:55 p.m. End of Meeting

Last updated: July 13, 2021