Sharon L. Milgram, Ph.D.
Dr. Milgram studies cell signaling and protein trafficking in polarized cells, including kidney and airway epithelial cells. Epithelial cells form a lining at the surface of the skin and along membranes within the body. This lining is essential for cell defense, nutrient absorption and ion transport. Her research group investigates how the topmost (or apical) membrane receptors regulate the activity of ion channels, including the epithelial sodium channel (ENaC) and the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel.
Focused initially on the airway epithelium, Dr. Milgram's research program has now expanded to study other epithelial cells and model systems, including the kidney and gastrointestinal tract. Her laboratory's findings suggest that receptors, signaling intermediates and effectors are compartmentalized into regulatory complexes that increase the fidelity and efficiency of cell signaling. These studies utilize diverse approaches, ranging from in vitro biochemical assays to physiological assays in knock-out mice.
Altschuler Y, Hodson C, Milgram SL. The apical compartment: trafficking pathways, regulators and scaffolding proteins. Curr Op Cell Biol, 15:423-429. 2003. [PubMed]
Smith PM, Cowan A, Milgram SL, White BA. Tissue-specific regulation by estrogen of ezrin and ezrin/radixin/moesin-binding protein 50. Endocrine, 22:119-126. 2003. [PubMed]
Barnes AP, Smith DF, VanDongen HM, VanDongen AMJ, Milgram SL. The identification of a second actin-binding region in spinophilin/neurabin II. Mol Brain Res, 124:105-113. 2004. [PubMed]
McHugh EM, Zhu W, Milgram S, Mager S. The GABA transporter GAT1 and the MUGUK protein PALS1: interaction, uptake modulation and co-expression in the brain. Cell Mol Neurosci, 26:406-417. 2004. [PubMed]
Howell M, Borchers CH, Milgram SL. hnRNP U interacts with Yes-Associated Protein (YAP65) and regulates its co-activation of p73-mediated Bax transcription. J Biol Chem, 279:26300-26306. 2004. [PubMed]
Huang P, Gilmore E, Kultgen P, Barnes AP, Milgram S, and Stutts MJ. Local regulation of cystic fibrosis transmembrane regulator and epithelial sodium channels in airway epithelium. Proc Am Thor Soc, 1:33-37. 2004. [PubMed]
Barnes AP, Livera G, Huang P, O'Neal W, Conti, M, Stutts MJ, Milgram SL. Phosphodiesterase 4 forms a cAMP diffusion barrier and a source for AMP signaling at the apical membrane of airway epithelium. J Biol Chem, 280:7997-8003. 2005. [PubMed]
Wang X, Zeng W, Soyombo AA, Tang W, Ross EM, Barnes AP, Milgram SL, Penninger JM, Allen PB, Greengard P, and Muallem S. Spinophilin regulates Ca2+ signaling by binding the N-terminal domain of RGS2 and the third intracellular loop of GPCRs. Nature Cell Biol, 7:405-411. 2005. [PubMed]
Thelin WR, Hodson CA, Milgram SL. Beyond the brush border: NHERF4 blazes new NHERF turf. J Physiol, 567:13-19. 2005. [PubMed]
Donowitz M, Milgram S, Murer H, Yun C, and Weinman E. Coming out of the NHERF family. J Physiol, 567: 1-6. 2005. [Full Text]
Morin-Kensicki E, Boone BN, Howell M, Stonebraker J, Alb J, Magnuson T, O'Neal W, and Milgram SL. Defects in yolk sac vasculogenesis, chorioallantoic fusion, and embryonic axis elongation in mice with targeted disruption of Yap65. Mol Cell Biol, 26:77-87. 2006. [PubMed]
Hodson CA, Ambogi IG, Scott RO, Mohler PJ, Milgram SL. Polarized apical sorting of guanylyl cyclase C is specified by a cytosolic signal. Traffic, 7:456-464. 2006. [PubMed]
Chen Y, Thelin WR, Yang B, Milgram S, and Jacobson K. Transient anchorage of cross-linked GPI-anchored proteins depends on cholesterol, Src family kinases, caveolin, and phosphoinositides. J Cell Biol, 175:169-178. 2006. [PubMed]
Thelin WR, Chen Y, Gentzch M, Kreda S, Borschers C, Jacobson K, Stutts MJ, Milgram SL. Direct interaction with filamins modulates the stability and plasma membrane expression of CFTR. J Clin Invest, 117: 364-74. 2007. [PubMed]