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NIH

Advanced Genomic Technology Development Meeting

May 30 - June 1, 2018
Northeastern University
East Village 17th Floor Event Space
360 Huntington Avenue
Boston, Massachusetts 02115

DNA Sequencing

On May 30 - June 1, 2018, the National Human Genome Research Institute (NHGRI) hosted the Advanced Genomic Technology Development Meeting at Northeastern University in Boston, Massachusetts. The purpose of the meeting was to provide an open forum on key scientific and technical challenges to advancing a broad set of genomic technologies including those for nucleic acid sequencing. 

The meeting brought together NHGRI Genomic Technology Program grantees and other scientists and engineers who had insights that would help to achieve the goal of sequencing human genomes at high quality for much less than $1,000, and develop a broad suite of new genomic technologies.

NHGRI Extramural Training Opportunities PDF file
NHGRI Strategic Planning Session Summary  PDF file

Wednesday, May 30, 2018

7:30 a.m.

Breakfast and Registration

8:30 a.m.

Welcome
Mike Smith and Carolyn Hutter

Session Chair: Mike Pazin (NHGRI)

8:50 a.m.

Towards high accuracy nanopore sequencing
Jens Gundlach

9:10 a.m.

Modeling macromolecular transport through protein channels and nanopores
Murugappan Muthukumar 

9:30 a.m.

Enzyme-less DNA base discrimination using solid-state nanopores with high-frequency integrated detection electronics
Kenneth Shepard

9:50 a.m.

Towards single-molecule RNA sequencing by recognition tunneling
Stuart Lindsay

10:10 a.m.

Porous zero-mode waveguides for single-molecule sequencing
Meni Wanunu

10:30 a.m.

BREAK

 

Session Chair: Paul Blainey

10:55 a.m.

Reflecting forward
Jeff Schloss

11:15 a.m.

Spatiogenetic imaging with DNA microscopy
Joshua Weinstein

11:35 a.m.

Imaging and sequencing the epigenome in situ
Jason Buenrostro

11:55 a.m.

All-optical genetic screens
David Feldman

12:15 p.m.

Lightning Talks (Noakes, Laszlo, Jou, Payne, Jensen, Yusuf, Padhy, Gabriel, Trotta, Alden): Wanunu Chair

12:30 p.m.

Lunch and Posters

Session Chair: Chuner Guo (Morris)

1:40 p.m.

Surveying transcription factor pioneer interactions with nucleosomal DNA
Luca Mariani

2:00 p.m.

Ultra high-throughput DNA synthesis via nano-optical conveyer belts
Mike Jensen/Punnag Padhy

2:20 p.m.

Low-input and high-throughput microfluidic technology for chip-seq with 30-100 cells per assay
Chang Lu 

2:40 p.m.

Combinatorial crispr screening of genetic interactions in breast cancer progression
Xiaoyu Zhao

3:00 p.m.

Constructing carbon nanotube transistors and measuring the activity of single-molecule DNA polymerases
Gregory Weiss

3:20 p.m.

Rapid, high-throughput and automation-friendly DNA prep kit for generating chromosome-spanning linked reads from conventional short-read sequencing
Siddharth Selvaraj

3:40 p.m.

BREAK

Session Chair: Winston Timp

4:10 p.m.

Lead into discussion: Balancing science and your "real" life
Samantha Morris
Andrew Laszlo (Gundlach)

Discussion Q&A
Sadhana Chalise (Muthukumar)

5:15 p.m.

Northeastern Reception

 

Thursday, May 31, 2018

7:30 a.m.

Breakfast

Session Chair: Henry Brinkerhoff (Gundlach)

8:30 a.m.

Improving read length, accuracy and availability of single-molecule DNA sequencing
Henk Postma

8:45 a.m.

Nanopore-based applications for labelled RNAs
Tessi Kanavarioti

9:00 a.m.

Maximizing spatial resolution of DNA sequencing using single carbon chain
Steve Tung

9:15 a.m.

DNA sequencing with two-dimensional nanopores and multiplexed CMOS electronics 
David Nidezwiecki

9:30 a.m.

Standards in genomic technology: what do we need over the next 5 years
Mark Salit

9:55 a.m.

BREAK

Session Chair: Sachi Inukai (Bulyk)

10:15 a.m.

Single-cell analysis of clonal dynamics in direct lineage reprogramming: a combinatorial indexing method for lineage tracing 
Samantha Morris

10:35 a.m.

Mapping cellular reprogramming via pooled overexpression screens with paired fitness and single cell RNA-sequencing readout
Prashant Mali

10:55 a.m.

Single-cell and single-molecule detection of epigenetic modifications
Dan Jones

11:15 a.m.

Cite-Seq and related technologies for high content single cell phenotyping
Peter Smimbert

11:35 a.m.

ChIP in snap: qualitative/quantitative ChIP control with defined sets of spike-in nucleosomal standards
Michael-Christopher Keogh

11:55 a.m.

Linear assembly of a human centromere on the Y chromosome
Karen Miga

12:15 p.m.

Lunch

Session Chair: Amy Jou (Muthukumar)

1:10 p.m.

Harnessing RNA targeting CRISPR systems for transcriptome engineering and human health
Omar Abudayyeh

1:30 p.m.

Development of lc/ms-based direct RNA sequencing with concomitant base-calling and modification analysis capability
Shenglong Zhang

1:50 p.m.

Whole-genome methylation profiling by methyltransferase-mediated hypermodification of DNA
Timothy Bestor

2:10 p.m.

Applications of modification in nanopore sequencing 
Winston Timp

2:30 p.m.

NHGRI Extramural Training Opportunities PDF file
Tina Gatlin

2:50 p.m.

Lightning Talks (Parekh, Wu, Kim, Stephenson, Fan, Lee, Drexler, Pud, Luo, Guo, Liu): Wanunu Chair

3:05 p.m.

Group Photo, Break and Posters

Session Chair: Peter Smibert

4:25 p.m.

Lead into discussion: Building a successful career
Karen Miga (Akeson)
Jacquelyn DuVall (Trotta)

Discussion Q&A
Avtar Singh (Blainey)

5:30 p.m.

Northeastern Reception

 

Friday, June 1, 2018

7:30 a.m.

Breakfast

8:00 a.m.

Welcome: Carolyn Hutter and Mike Smith

Session Chair: Matthew Noakes (Gundlach)

8:15 a.m.

Chemical-crosslinking assisted proximity capture (CAP-C)
Chuan He

8:45 a.m.

Multi-omic analysis of single cells
Joe Ecker 

9:15 a.m.

Nanopore sequencing of native human poly(A) RNA 
Mark Akeson

9:45 a.m.

Nanotechnology for studying biology at the single-molecule level
Cees Dekker

10:15 a.m.

BREAK

Session Chair: Bo Ma (Tung)

10:35 a.m.

Exploring the nascent transcriptome with direct RNA nanopore sequencing
Stirling Churchman

11:05 a.m.

Comprehensive functional characterization of noncoding regulatory elements and genetic variation
Ryan Tewhey

11:35 a.m.

Complexity in TF-DNA binding and cis-regulatory element function
Martha Bulyk

12:05 p.m.

New frontiers for pooled screens: finding regulatory elements in the noncoding genome and capturing multi-cell interactions
Neville Sanjana

12:35 p.m.

Lunch and Meeting Feedback

1:25 p.m.

NHGRI Strategic Planning Session
Carolyn Hutter, Mike Pazin, Mike Smith

2:55 p.m.

Break and Posters

Session Chair: Mohammad Amin Alibakhshi (Wanunu)

3:55 p.m.

Multi-scale 3D genome mapping of human iPSC and neuron differentiation with easy Hi-C
Fulai Jin

4:10 p.m.

Next-generation genomic target enrichment - amplification-free capture and sequencing of large chromosomal segments
Johannes Dapprich

4:25 p.m.

Nanobind magnetic disks for rapid high MW DNA extraction and long-read sequencing library preparation
Kelvin Liu

4:40 p.m.

Adjourn

Last Updated: September 27, 2018