2018 Advanced Genomic Technology Development Meeting

Event Details

The National Human Genome Research Institute (NHGRI) hosted the Advanced Genomic Technology Development Meeting at Northeastern University in Boston, Massachusetts, on May 30 - June 1, 2018. The purpose of the meeting was to provide an open forum on key scientific and technical challenges to advancing a broad set of genomic technologies including those for nucleic acid sequencing. 

The meeting brought together NHGRI Genomic Technology Program grantees and other scientists and engineers who had insights that would help to achieve the goal of sequencing human genomes at high quality for much less than $1,000, and develop a broad suite of new genomic technologies.

to

Northeastern University
East Village 17th Floor Event Space
360 Huntington Avenue
Boston, Massachusetts 02115

Agenda

  • Wednesday, May 30, 2018

  • 7:30 a.m. Breakfast and Registration
  • 8:30 a.m. Welcome
    Mike Smith and Carolyn Hutter
  • Session Chair: Mike Pazin (NHGRI)
  • 8:50 a.m. Towards high accuracy nanopore sequencing
    Jens Gundlach
  • 9:10 a.m. Modeling macromolecular transport through protein channels and nanopores
    Murugappan Muthukumar
  • 9:30 a.m. Enzyme-less DNA base discrimination using solid-state nanopores with high-frequency integrated detection electronics
    Kenneth Shepard
  • 9:50 a.m. Towards single-molecule RNA sequencing by recognition tunneling
    Stuart Lindsay
  • 10:10 a.m. Porous zero-mode waveguides for single-molecule sequencing
    Meni Wanunu
  • 10:30 a.m. BREAK
  • Session Chair: Paul Blainey
  • 10:55 a.m. Reflecting forward
    Jeff Schloss
  • 11:15 a.m. Spatiogenetic imaging with DNA microscopy
    Joshua Weinstein
  • 11:35 a.m. Imaging and sequencing the epigenome in situ
    Jason Buenrostro
  • 11:55 a.m. All-optical genetic screens
    David Feldman
  • 12:15 p.m. Lightning Talks (Noakes, Laszlo, Jou, Payne, Jensen, Yusuf, Padhy, Gabriel, Trotta, Alden): Wanunu Chair
  • 12:30 p.m. Lunch and Posters
  • Session Chair: Chuner Guo (Morris)
  • 1:40 p.m. Surveying transcription factor pioneer interactions with nucleosomal DNA
    Luca Mariani
  • 2:00 p.m. Ultra high-throughput DNA synthesis via nano-optical conveyer belts
    Mike Jensen/Punnag Padhy
  • 2:20 p.m. Low-input and high-throughput microfluidic technology for chip-seq with 30-100 cells per assay
    Chang Lu
  • 2:40 p.m. Combinatorial crispr screening of genetic interactions in breast cancer progression
    Xiaoyu Zhao
  • 3:00 p.m. Constructing carbon nanotube transistors and measuring the activity of single-molecule DNA polymerases
    Gregory Weiss
  • 3:20 p.m. Rapid, high-throughput and automation-friendly DNA prep kit for generating chromosome-spanning linked reads from conventional short-read sequencing
    Siddharth Selvaraj
  • 3:40 p.m. BREAK
  • Session Chair: Winston Timp
  • 4:10 p.m. Lead into discussion: Balancing science and your "real" life
    Samantha Morris
    Andrew Laszlo (Gundlach)
  • Discussion Q&A
    Sadhana Chalise (Muthukumar)
  • 5:15 p.m. Northeastern Reception

 

  • Thursday, May 31, 2018

  • 7:30 a.m. Breakfast
  • Session Chair: Henry Brinkerhoff (Gundlach)
  • 8:30 a.m. Improving read length, accuracy and availability of single-molecule DNA sequencing
    Henk Postma
  • 8:45 a.m. Nanopore-based applications for labelled RNAs
    Tessi Kanavarioti
  • 9:00 a.m. Maximizing spatial resolution of DNA sequencing using single carbon chain
    Steve Tung
  • 9:15 a.m. DNA sequencing with two-dimensional nanopores and multiplexed CMOS electronics
    David Nidezwiecki
  • 9:30 a.m. Standards in genomic technology: what do we need over the next 5 years
    Mark Salit
  • 9:55 a.m. BREAK
  • Session Chair: Sachi Inukai (Bulyk)
  • 10:15 a.m. Single-cell analysis of clonal dynamics in direct lineage reprogramming: a combinatorial indexing method for lineage tracing
    Samantha Morris
  • 10:35 a.m. Mapping cellular reprogramming via pooled overexpression screens with paired fitness and single cell RNA-sequencing readout
    Prashant Mali
  • 10:55 a.m. Single-cell and single-molecule detection of epigenetic modifications
    Dan Jones
  • 11:15 a.m. Cite-Seq and related technologies for high content single cell phenotyping
    Peter Smimbert
  • 11:35 a.m. ChIP in snap: qualitative/quantitative ChIP control with defined sets of spike-in nucleosomal standards
    Michael-Christopher Keogh
  • 11:55 a.m. Linear assembly of a human centromere on the Y chromosome
    Karen Miga
  • 12:15 p.m. Lunch
  • Session Chair: Amy Jou (Muthukumar)
  • 1:10 p.m. Harnessing RNA targeting CRISPR systems for transcriptome engineering and human health
    Omar Abudayyeh
  • 1:30 p.m. Development of lc/ms-based direct RNA sequencing with concomitant base-calling and modification analysis capability
    Shenglong Zhang
  • 1:50 p.m. Whole-genome methylation profiling by methyltransferase-mediated hypermodification of DNA
    Timothy Bestor
  • 2:10 p.m. Applications of modification in nanopore sequencing
    Winston Timp
  • 2:30 p.m. NHGRI Extramural Training Opportunities
    Tina Gatlin
  • 2:50 p.m. Lightning Talks (Parekh, Wu, Kim, Stephenson, Fan, Lee, Drexler, Pud, Luo, Guo, Liu): Wanunu Chair
  • 3:05 p.m. Group Photo, Break and Posters
  • Session Chair: Peter Smibert
  • 4:25 p.m. Lead into discussion: Building a successful career
    Karen Miga (Akeson)
    Jacquelyn DuVall (Trotta)
  • Discussion Q&A
    Avtar Singh (Blainey)
  • 5:30 p.m. Northeastern Reception

 

  • Friday, June 1, 2018

  • 7:30 a.m. Breakfast
  • 8:00 a.m. Welcome: Carolyn Hutter and Mike Smith
  • Session Chair: Matthew Noakes (Gundlach)
  • 8:15 a.m. Chemical-crosslinking assisted proximity capture (CAP-C)
    Chuan He
  • 8:45 a.m. Multi-omic analysis of single cells
    Joe Ecker
  • 9:15 a.m. Nanopore sequencing of native human poly(A) RNA
    Mark Akeson
  • 9:45 a.m. Nanotechnology for studying biology at the single-molecule level
    Cees Dekker
  • 10:15 a.m. BREAK
  • Session Chair: Bo Ma (Tung)
  • 10:35 a.m. Exploring the nascent transcriptome with direct RNA nanopore sequencing
    Stirling Churchman
  • 11:05 a.m. Comprehensive functional characterization of noncoding regulatory elements and genetic variation
    Ryan Tewhey
  • 11:35 a.m. Complexity in TF-DNA binding and cis-regulatory element function
    Martha Bulyk
  • 12:05 p.m. New frontiers for pooled screens: finding regulatory elements in the noncoding genome and capturing multi-cell interactions
    Neville Sanjana
  • 12:35 p.m. Lunch and Meeting Feedback
  • 1:25 p.m. NHGRI Strategic Planning Session
    Carolyn Hutter, Mike Pazin, Mike Smith

    NHGRI Strategic Planning Session Summary
  • 2:55 p.m. Break and Posters
  • Session Chair: Mohammad Amin Alibakhshi (Wanunu)
  • 3:55 p.m. Multi-scale 3D genome mapping of human iPSC and neuron differentiation with easy Hi-C
    Fulai Jin
  • 4:10 p.m. Next-generation genomic target enrichment - amplification-free capture and sequencing of large chromosomal segments
    Johannes Dapprich
  • 4:25 p.m. Nanobind magnetic disks for rapid high MW DNA extraction and long-read sequencing library preparation
    Kelvin Liu
  • 4:40 p.m. Adjourn

Last updated: September 27, 2018