2018 Advanced Genomic Technology Development Meeting
Event Details
The National Human Genome Research Institute (NHGRI) hosted the Advanced Genomic Technology Development Meeting at Northeastern University in Boston, Massachusetts, on May 30 - June 1, 2018. The purpose of the meeting was to provide an open forum on key scientific and technical challenges to advancing a broad set of genomic technologies including those for nucleic acid sequencing.
The meeting brought together NHGRI Genomic Technology Program grantees and other scientists and engineers who had insights that would help to achieve the goal of sequencing human genomes at high quality for much less than $1,000, and develop a broad suite of new genomic technologies.
to
Northeastern University
East Village 17th Floor Event Space
360 Huntington Avenue
Boston, Massachusetts 02115
Agenda
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Wednesday, May 30, 2018
- 7:30 a.m. Breakfast and Registration
- 8:30 a.m. Welcome
Mike Smith and Carolyn Hutter - Session Chair: Mike Pazin (NHGRI)
- 8:50 a.m. Towards high accuracy nanopore sequencing
Jens Gundlach - 9:10 a.m. Modeling macromolecular transport through protein channels and nanopores
Murugappan Muthukumar - 9:30 a.m. Enzyme-less DNA base discrimination using solid-state nanopores with high-frequency integrated detection electronics
Kenneth Shepard - 9:50 a.m. Towards single-molecule RNA sequencing by recognition tunneling
Stuart Lindsay - 10:10 a.m. Porous zero-mode waveguides for single-molecule sequencing
Meni Wanunu - 10:30 a.m. BREAK
- Session Chair: Paul Blainey
- 10:55 a.m. Reflecting forward
Jeff Schloss - 11:15 a.m. Spatiogenetic imaging with DNA microscopy
Joshua Weinstein - 11:35 a.m. Imaging and sequencing the epigenome in situ
Jason Buenrostro - 11:55 a.m. All-optical genetic screens
David Feldman - 12:15 p.m. Lightning Talks (Noakes, Laszlo, Jou, Payne, Jensen, Yusuf, Padhy, Gabriel, Trotta, Alden): Wanunu Chair
- 12:30 p.m. Lunch and Posters
- Session Chair: Chuner Guo (Morris)
- 1:40 p.m. Surveying transcription factor pioneer interactions with nucleosomal DNA
Luca Mariani - 2:00 p.m. Ultra high-throughput DNA synthesis via nano-optical conveyer belts
Mike Jensen/Punnag Padhy - 2:20 p.m. Low-input and high-throughput microfluidic technology for chip-seq with 30-100 cells per assay
Chang Lu - 2:40 p.m. Combinatorial crispr screening of genetic interactions in breast cancer progression
Xiaoyu Zhao - 3:00 p.m. Constructing carbon nanotube transistors and measuring the activity of single-molecule DNA polymerases
Gregory Weiss - 3:20 p.m. Rapid, high-throughput and automation-friendly DNA prep kit for generating chromosome-spanning linked reads from conventional short-read sequencing
Siddharth Selvaraj - 3:40 p.m. BREAK
- Session Chair: Winston Timp
- 4:10 p.m. Lead into discussion: Balancing science and your "real" life
Samantha Morris
Andrew Laszlo (Gundlach) - Discussion Q&A
Sadhana Chalise (Muthukumar) - 5:15 p.m. Northeastern Reception
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Thursday, May 31, 2018
- 7:30 a.m. Breakfast
- Session Chair: Henry Brinkerhoff (Gundlach)
- 8:30 a.m. Improving read length, accuracy and availability of single-molecule DNA sequencing
Henk Postma - 8:45 a.m. Nanopore-based applications for labelled RNAs
Tessi Kanavarioti - 9:00 a.m. Maximizing spatial resolution of DNA sequencing using single carbon chain
Steve Tung - 9:15 a.m. DNA sequencing with two-dimensional nanopores and multiplexed CMOS electronics
David Nidezwiecki - 9:30 a.m. Standards in genomic technology: what do we need over the next 5 years
Mark Salit - 9:55 a.m. BREAK
- Session Chair: Sachi Inukai (Bulyk)
- 10:15 a.m. Single-cell analysis of clonal dynamics in direct lineage reprogramming: a combinatorial indexing method for lineage tracing
Samantha Morris - 10:35 a.m. Mapping cellular reprogramming via pooled overexpression screens with paired fitness and single cell RNA-sequencing readout
Prashant Mali - 10:55 a.m. Single-cell and single-molecule detection of epigenetic modifications
Dan Jones - 11:15 a.m. Cite-Seq and related technologies for high content single cell phenotyping
Peter Smimbert - 11:35 a.m. ChIP in snap: qualitative/quantitative ChIP control with defined sets of spike-in nucleosomal standards
Michael-Christopher Keogh - 11:55 a.m. Linear assembly of a human centromere on the Y chromosome
Karen Miga - 12:15 p.m. Lunch
- Session Chair: Amy Jou (Muthukumar)
- 1:10 p.m. Harnessing RNA targeting CRISPR systems for transcriptome engineering and human health
Omar Abudayyeh - 1:30 p.m. Development of lc/ms-based direct RNA sequencing with concomitant base-calling and modification analysis capability
Shenglong Zhang - 1:50 p.m. Whole-genome methylation profiling by methyltransferase-mediated hypermodification of DNA
Timothy Bestor - 2:10 p.m. Applications of modification in nanopore sequencing
Winston Timp - 2:30 p.m. NHGRI Extramural Training Opportunities
Tina Gatlin - 2:50 p.m. Lightning Talks (Parekh, Wu, Kim, Stephenson, Fan, Lee, Drexler, Pud, Luo, Guo, Liu): Wanunu Chair
- 3:05 p.m. Group Photo, Break and Posters
- Session Chair: Peter Smibert
- 4:25 p.m. Lead into discussion: Building a successful career
Karen Miga (Akeson)
Jacquelyn DuVall (Trotta) - Discussion Q&A
Avtar Singh (Blainey) - 5:30 p.m. Northeastern Reception
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Friday, June 1, 2018
- 7:30 a.m. Breakfast
- 8:00 a.m. Welcome: Carolyn Hutter and Mike Smith
- Session Chair: Matthew Noakes (Gundlach)
- 8:15 a.m. Chemical-crosslinking assisted proximity capture (CAP-C)
Chuan He - 8:45 a.m. Multi-omic analysis of single cells
Joe Ecker - 9:15 a.m. Nanopore sequencing of native human poly(A) RNA
Mark Akeson - 9:45 a.m. Nanotechnology for studying biology at the single-molecule level
Cees Dekker - 10:15 a.m. BREAK
- Session Chair: Bo Ma (Tung)
- 10:35 a.m. Exploring the nascent transcriptome with direct RNA nanopore sequencing
Stirling Churchman - 11:05 a.m. Comprehensive functional characterization of noncoding regulatory elements and genetic variation
Ryan Tewhey - 11:35 a.m. Complexity in TF-DNA binding and cis-regulatory element function
Martha Bulyk - 12:05 p.m. New frontiers for pooled screens: finding regulatory elements in the noncoding genome and capturing multi-cell interactions
Neville Sanjana - 12:35 p.m. Lunch and Meeting Feedback
- 1:25 p.m. NHGRI Strategic Planning Session
Carolyn Hutter, Mike Pazin, Mike Smith
NHGRI Strategic Planning Session Summary - 2:55 p.m. Break and Posters
- Session Chair: Mohammad Amin Alibakhshi (Wanunu)
- 3:55 p.m. Multi-scale 3D genome mapping of human iPSC and neuron differentiation with easy Hi-C
Fulai Jin - 4:10 p.m. Next-generation genomic target enrichment - amplification-free capture and sequencing of large chromosomal segments
Johannes Dapprich - 4:25 p.m. Nanobind magnetic disks for rapid high MW DNA extraction and long-read sequencing library preparation
Kelvin Liu - 4:40 p.m. Adjourn
Last updated: September 27, 2018